Piperine inhibits ABCA1 degradation and promotes cholesterol efflux from THP-1-derived macrophages.

ABSTRACT:

Scope

Increased macrophage cholesterol efflux (ChE) is considered to have anti-atherosclerotic effect counteracting cardiovascular disease. The principle pungent ingredient of the fruits of Piper nigrum, piperine, is identified in this study as a ChE inducer in THP-1-derived macrophages, and mechanisms underlying this effect are explored.

Methods and results

Without affecting cell viability, piperine concentration-dependently enhances ChE in THP-1-derived macrophages from 25 to 100 μM. The expression level of the key cholesterol transporter protein ATP-binding cassette transporter A1 (ABCA1) is significantly upregulated by piperine, as revealed by western blot analyses. However, two other ChE-mediating transporter proteins, ATP-binding cassette transporter G1 (ABCG1) and sca

SUBMITTER: Wang L 

PROVIDER: S-EPMC5382977 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature