Cell-Type-Specific Optical Recording of Membrane Voltage Dynamics in Freely Moving Mice.

ABSTRACT: Electrophysiological field potential dynamics are of fundamental interest in basic and clinical neuroscience, but how specific cell types shape these dynamics in the live brain is poorly understood. To empower mechanistic studies, we created an optical technique, TEMPO, that records the aggregate trans-membrane voltage dynamics of genetically specified neurons in freely behaving mice. TEMPO has >10-fold greater sensitivity than prior fiber-optic techniques and attains the noise minimum set by quantum mechanical photon shot noise. After validating TEMPO's capacity to track established oscillations in the delta, theta, and gamma frequency bands, we compared the D1- and D2-dopamine-receptor-expressing striatal medium spiny neurons (MSNs), which are interspersed and electrically indistinguisha

SUBMITTER: Marshall JD 

PROVIDER: S-EPMC5382987 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature