Structural and molecular analysis of a protective epitope of Lyme disease antigen OspA and antibody interactions.

ABSTRACT: The murine monoclonal antibody LA-2 recognizes a clinically protective epitope on outer surface protein (OspA) of Borrelia burgdorferi, the causative agent of Lyme disease in North America. Human antibody equivalence to LA-2 is the best serologic correlate of protective antibody responses following OspA vaccination. Understanding the structural and functional basis of the LA-2 protective epitope is important for developing OspA-based vaccines and discovering prophylactic antibodies against Lyme disease. Here, we present a detailed structure-based analysis of the LA-2/OspA interaction interface and identification of residues mediating antibody recognition. Mutations were introduced into both OspA and LA-2 on the basis of computational predictions on the crystal structure of the complex and

SUBMITTER: Shandilya S 

PROVIDER: S-EPMC5383521 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature