Vaccination with poly(IC:LC) and peptide-pulsed autologous dendritic cells in patients with pancreatic cancer.

ABSTRACT:

Background

Dendritic cells (DCs) enhance the quality of anti-tumor immune response in patients with cancer. Thus, we posit that DC-based immunotherapy, in conjunction with toll-like receptor (TLR)-3 agonist poly-ICLC, is a promising approach for harnessing immunity against metastatic or locally advanced unresectable pancreatic cancer (PC).

Methods

We generated autologous DCs from the peripheral blood of HLA-A2⁺ patients with PC. DCs were pulsed with three distinct A2-restricted peptides: 1) human telomerase reverse transcriptase (hTERT, TERT572Y), 2) carcinoembryonic antigen (CEA; Cap1-6D), and 3) survivin (SRV.A2). Patients received four intradermal injections of 1 × 10⁷ peptide-pulsed DC vaccines every 2 weeks (Day 0, 14, 28, and 42). Concurrently, pa

SUBMITTER: Mehrotra S 

PROVIDER: S-EPMC5384142 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature