A robust and cost-effective approach to sequence and analyze complete genomes of small RNA viruses.

ABSTRACT:

Background

Next-generation sequencing (NGS) allows ultra-deep sequencing of nucleic acids. The use of sequence-independent amplification of viral nucleic acids without utilization of target-specific primers provides advantages over traditional sequencing methods and allows detection of unsuspected variants and co-infecting agents. However, NGS is not widely used for small RNA viruses because of incorrectly perceived cost estimates and inefficient utilization of freely available bioinformatics tools.

Methods

In this study, we have utilized NGS-based random sequencing of total RNA combined with barcode multiplexing of libraries to quickly, effectively and simultaneously characterize the genomic sequences of multiple avian paramyxoviruses. Thirty libraries were prepared from di

SUBMITTER: Dimitrov KM 

PROVIDER: S-EPMC5384157 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature