APMicroDB: A microsatellite database of Acyrthosiphon pisum.
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ABSTRACT: Pea aphids represent a complex genetic system that could be used for QTL analysis, genetic diversity and population genetics studies. Here, we described the development of first microsatellite repeat database of the pea aphid (APMicroDB), accessible at "http://deepaklab.com/aphidmicrodb". We identified 3,40,233 SSRs using MIcroSAtellite (MISA) tool that was distributed in 14,067 (out of 23,924) scaffold of the pea aphid. We observed 89.53% simple repeats of which 73.41% were mono-nucleotide, followed by di-nucleotide repeats. This database stored information about the repeats kind, GC content, motif type (mono - hexa), genomic location etc. We have also incorporated the primer information derived from Primer3 software of the 2504 bp flanking region of the identified marker. Blast tool is a
SUBMITTER: Bishnoi R
PROVIDER: S-EPMC5384296 | biostudies-literature | 2017 Jun
REPOSITORIES: biostudies-literature
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