Exosomal transfer from human renal proximal tubule cells to distal tubule and collecting duct cells.

ABSTRACT:

Objectives

Exosomes are 50-90nm extracellular membrane particles that may mediate trans-cellular communication between cells and tissues. We have reported that human urinary exosomes contain miRNA that are biomarkers for salt sensitivity and inverse salt sensitivity of blood pressure. This study examines exosomal transfer between cultured human renal proximal tubule cells (RPTCs) and from RPTCs to human distal tubule and collecting duct cells.

Design and methods

For RPTC-to-RPTC exosomal transfer, we utilized 5 RPTC lines producing exosomes that were fluorescently labeled with exosomal-specific markers CD63-EGFP or CD9-RFP. Transfer between RPTCs was demonstrated by co-culturing CD63-EGFP and CD9-RFP stable clones and performing live confocal microscopy. For RPTC-to-distal s

SUBMITTER: Gildea JJ 

PROVIDER: S-EPMC5384615 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature