A strategy to identify linker-based modules for the allosteric regulation of antibody-antigen binding affinities of different scFvs.

ABSTRACT: Antibody single-chain variable fragments (scFvs) are used in a variety of applications, such as for research, diagnosis and therapy. Essential for these applications is the extraordinary specificity, selectivity and affinity of antibody paratopes, which can also be used for efficient protein purification. However, this use is hampered by the high affinity for the protein to be purified because harsh elution conditions, which may impair folding, integrity or viability of the eluted biomaterials, are typically required. In this study, we developed a strategy to obtain structural elements that provide allosteric modulation of the affinities of different antibody scFvs for their antigen. To identify suitable allosteric modules, a complete set of cyclic permutations of calmodulin variants was g

SUBMITTER: Kellmann SJ 

PROVIDER: S-EPMC5384732 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature