Skin microbiome promotes mast cell maturation by triggering stem cell factor production in keratinocytes.

ABSTRACT:

Background

Mast cell (MC) progenitors leave the bone marrow, enter the circulation, and settle in the skin and other tissues. Their maturation in tissues is influenced by the surrounding microenvironment.

Objective

We tested the hypothesis that environmental factors play a role in MC maturation in the skin.

Methods

MCs were numerically, phenotypically, and functionally compared between germ-free (GF), specific pathogen-free, and GF mice reconstituted with microbiota. The maturity of MCs was then correlated with skin levels of stem cell factor (SCF), a critical MC differentiation factor, and lipoteichoic acid (LTA), a Toll-like receptor 2 ligand. MCs were also evaluated in mice with keratinocyte-specific deletion of Scf.

Results

We found that GF mice express abn

SUBMITTER: Wang Z 

PROVIDER: S-EPMC5385284 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature