Rubisco Activases: AAA+ Chaperones Adapted to Enzyme Repair.

ABSTRACT: Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), the key enzyme of the Calvin-Benson-Bassham cycle of photosynthesis, requires conformational repair by Rubisco activase for efficient function. Rubisco mediates the fixation of atmospheric CO₂ by catalyzing the carboxylation of the five-carbon sugar ribulose-1,5-bisphosphate (RuBP). It is a remarkably inefficient enzyme, and efforts to increase crop yields by bioengineering Rubisco remain unsuccessful. This is due in part to the complex cellular machinery required for Rubisco biogenesis and metabolic maintenance. To function, Rubisco must undergo an activation process that involves carboxylation of an active site lysine by a non-substrate CO₂ molecule and binding of a Mg²⁺ ion. Premature binding of

SUBMITTER: Bhat JY 

PROVIDER: S-EPMC5385338 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature