Metabolomic characterisation of the effects of oncogenic PIK3CA transformation in a breast epithelial cell line.

ABSTRACT: Somatic mutations in PIK3CA are frequently found in a number of human cancers, including breast cancer, altering cellular physiology and tumour sensitivity to chemotherapy. This renders PIK3CA an attractive molecular target for early detection and personalised therapy. Using ¹H Nuclear Magnetic Resonance spectroscopy (NMR) and Gas Chromatography - Mass Spectrometery (GC-MS) together with ¹³C stable isotope-labelled glucose and glutamine as metabolic tracers, we probed the phenotypic changes in metabolism following a single copy knock-in of mutant PIK3CA (H1047R) in the MCF10A cell line, an important cell model for studying oncogenic transformation in breast tissues. We observed effects in several metabolic pathways, including a decrease in glycerophosphocholine level

SUBMITTER: Lau CE 

PROVIDER: S-EPMC5385542 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature