Chromatin dynamics regulate mesenchymal stem cell lineage specification and differentiation to osteogenesis.

ABSTRACT: Multipotent mesenchymal stromal cells (MSCs) are critical for regeneration of multiple tissues. Epigenetic mechanisms are fundamental regulators of lineage specification and cell fate, and as such, we addressed the question of which epigenetic modifications characterize the transition of nascent MSCs to a tissue specific MSC-derived phenotype. By profiling the temporal changes of seven histone marks correlated to gene expression during proliferation, early commitment, matrix deposition, and mineralization stages, we identified distinct epigenetic mechanisms that regulate transcriptional programs necessary for tissue-specific phenotype development. Patterns of stage-specific enrichment of histone modifications revealed distinct modes of repression and activation of gene expression that woul

SUBMITTER: Wu H 

PROVIDER: S-EPMC5385709 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature