Vamp-7-dependent secretion at the immune synapse regulates antigen extraction and presentation in B-lymphocytes.

ABSTRACT: Recognition of surface-tethered antigens (Ags) by B-cells leads to the formation of an immune synapse that promotes Ag uptake for presentation onto MHC-II molecules. Extraction of immobilized Ags at the immune synapse of B-cells relies on the local secretion of lysosomes, which are recruited to the Ag contact site by polarization of their microtubule network. Although conserved polarity proteins have been implicated in coordinating cytoskeleton remodeling with lysosome trafficking, the cellular machinery associated with lysosomal vesicles that regulates their docking and secretion at the synaptic interface has not been defined. Here we show that the v-SNARE protein Vamp-7 is associated with Lamp-1⁺ lysosomal vesicles, which are recruited and docked at the center of the immune sy

SUBMITTER: Obino D 

PROVIDER: S-EPMC5385938 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature