Transcriptomic variation of eyestalk reveals the genes and biological processes associated with molting in Portunus trituberculatus.

ABSTRACT:

Background

Molting is an essential biological process throughout the life history of crustaceans, which is regulated by many neuropeptide hormones expressed in the eyestalk. To better understand the molting mechanism in Portunus trituberculatus, we used digital gene expression (DGE) to analyze single eyestalk samples during the molting cycle by high-throughput sequencing.

Results

We obtained 14,387,942, 12,631,508 and 13,060,062 clean sequence reads from inter-molt (InM), pre-molt (PrM) and post-molt (PoM) cDNA libraries, respectively. A total of 1,394 molt-related differentially expressed genes (DEGs) were identified. GO and KEGG enrichment analysis identified some important processes and pathways with key roles in molting regulation, such as chitin metabolism, peptidase in

SUBMITTER: Lv J 

PROVIDER: S-EPMC5386282 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature