AIF-independent parthanatos in the pathogenesis of dry age-related macular degeneration.

ABSTRACT: Cell death of retinal pigment epithelium (RPE) is characterized as an essential late-stage phenomenon of dry age-related macular degeneration (AMD). The aim of this study was to elucidate the molecular mechanism underlying RPE cell death after exposure to oxidative stress, which occurs often because of the anatomical location of RPE cells. ARPE-19, an established RPE cell line, exhibited necrotic features involving poly (ADP-ribose) polymerase-1 (PARP-1) activation in response to hydrogen peroxide (H₂O₂). ARPE-19 cells were resistant to H₂O₂ when PARP-1 was depleted using siRNA or inhibited by a pharmacological inhibitor of PARP-1, olaparib. Our data suggest a causal relationship between PARP-1 activation and ARPE-19 cell death in response to H

SUBMITTER: Jang KH 

PROVIDER: S-EPMC5386356 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature