Deletion of Stk40 impairs definitive erythropoiesis in the mouse fetal liver.

ABSTRACT: The serine threonine kinase Stk40 has been shown to involve in mouse embryonic stem cell differentiation, pulmonary maturation and adipocyte differentiation. Here we report that targeted deletion of Stk40 leads to fetal liver hypoplasia and anemia in the mouse embryo. The reduction of erythrocytes in the fetal liver is accompanied by increased apoptosis and compromised erythroid maturation. Stk40^-/- fetal liver cells have significantly reduced colony-forming units (CFUs) capable of erythroid differentiation, including burst forming unit-erythroid, CFU-erythroid (CFU-E), and CFU-granulocyte, erythrocyte, megakaryocyte and macrophage, but not CFU-granulocyte/macrophages. Purified Stk40^-/- megakaryocyte-erythrocyte progenitors produce substantially fewer CFU-E colonies c

SUBMITTER: Wang L 

PROVIDER: S-EPMC5386544 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature