Stem cell cultures derived from pediatric brain tumors accurately model the originating tumors.

ABSTRACT: Brain tumors are the leading cause of cancer-related death in children but high-grade gliomas in children and adolescents have remained a relatively under-investigated disease despite this. A better understanding of the cellular and molecular pathogenesis of the diseases is required in order to improve the outcome for these children. In vitro-cultured primary tumor cells from patients are indispensable tools for this purpose by enabling functional analyses and development of new therapies. However, relevant well-characterized in vitro cultures from pediatric gliomas cultured under serum-free conditions have been lacking. We have therefore established patient-derived in vitro cultures and performed thorough characterization of the cells using large-scale analyses of DNA methylation, copy-nu

SUBMITTER: Wenger A 

PROVIDER: S-EPMC5386635 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature