Dendritic Cell-Specific Transmembrane Protein (DC-STAMP) Regulates Osteoclast Differentiation via the Ca²⁺ /NFATc1 Axis.

ABSTRACT: DC-STAMP is a multi-pass transmembrane protein essential for cell-cell fusion between osteoclast precursors during osteoclast (OC) development. DC-STAMP-/- mice have mild osteopetrosis and form mononuclear cells with limited resorption capacity. The identification of an Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM) on the cytoplasmic tail of DC-STAMP suggested a potential signaling function. The absence of a known DC-STAMP ligand, however, has hindered the elucidation of downstream signaling pathways. To address this problem, we engineered a light-activatable DC-STAMP chimeric molecule in which light exposure mimics ligand engagement that can be traced by downstream Ca²⁺ signaling. Deletion of the cytoplasmic ITIM resulted in a significant elevation in the amplitude and

SUBMITTER: Chiu YH 

PROVIDER: S-EPMC5386838 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature