Multiple roles for a novel RND-type efflux system in Acinetobacter baumannii AB5075.

ABSTRACT: Colony opacity phase variation in Acinetobacter baumannii strain AB5075 is regulated by a reversible high-frequency switch. Transposon mutagenesis was used to generate mutations that decreased the opaque to translucent switch and a gene encoding a predicted periplasmic membrane fusion component of a resistance-nodulation-cell division (RND)-type efflux system was isolated. This gene was designated arpA and immediately downstream was a gene designated arpB that encodes a predicted membrane transporter of RND-type systems. A nonpolar, in-frame deletion in arpA resulted in a 70-fold decrease in the opaque to translucent switch. An arpB::Tc mutant exhibited a 769-fold decrease in the opaque to translucent switch. However, the translucent to opaque switch was largely unchanged in both the arpA

SUBMITTER: Tipton KA 

PROVIDER: S-EPMC5387308 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature