Links between DNA methylation and nucleosome occupancy in the human genome.

ABSTRACT:

Background

DNA methylation is an epigenetic modification that is enriched in heterochromatin but depleted at active promoters and enhancers. However, the debate on whether or not DNA methylation is a reliable indicator of high nucleosome occupancy has not been settled. For example, the methylation levels of DNA flanking CTCF sites are higher in linker DNA than in nucleosomal DNA, while other studies have shown that the nucleosome core is the preferred site of methylation. In this study, we make progress toward understanding these conflicting phenomena by implementing a bioinformatics approach that combines MNase-seq and NOMe-seq data and by comprehensively profiling DNA methylation and nucleosome occupancy throughout the human genome.

Results

The results demonstrated that in

SUBMITTER: Collings CK 

PROVIDER: S-EPMC5387343 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature