Genetically encoded biosensors for visualizing live-cell biochemical activity at super-resolution.

ABSTRACT: Compartmentalized biochemical activities are essential to all cellular processes, but there is no generalizable method to visualize dynamic protein activities in living cells at a resolution commensurate with cellular compartmentalization. Here, we introduce a new class of fluorescent biosensors that detect biochemical activities in living cells at a resolution up to threefold better than the diffraction limit. These 'FLINC' biosensors use binding-induced changes in protein fluorescence dynamics to translate kinase activities or protein-protein interactions into changes in fluorescence fluctuations, which are quantifiable through stochastic optical fluctuation imaging. A protein kinase A (PKA) biosensor allowed us to resolve minute PKA activity microdomains on the plasma membranes of livin

SUBMITTER: Mo GC 

PROVIDER: S-EPMC5388356 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature