Electrochemical Impedance Spectroscopy Study of Concanavalin A Binding to Self-Assembled Monolayers of Mannosides on Gold Wire Electrodes.

ABSTRACT: The interactions of the lectin Concanavalin A (Con A) with self-assembled monolayers (SAMs) of thiolated mono-, di-, and tri-mannosides were studied on the surface of gold wires using electrochemical impedance spectroscopy (EIS). The SAMs of mannosides were prepared either pure or along with thiolated triethylene glycol (TEG) at different molar ratios (1:1, 1:2, 1:4, 1:9, and 1:19) to better understand and optimize the interaction conditions. The charge-transfer resistance of the [Fe(CN)₆]^3-/^4- redox probe was compared before and after the interaction at different concentrations of Con A to determine the equilibrium dissociation constant (K_d) and limit of detection (LOD). Values of K_d were found in the nanomolar range sho

SUBMITTER: Bhattarai JK 

PROVIDER: S-EPMC5388449 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature