Crossing kingdoms: Using decellularized plants as perfusable tissue engineering scaffolds.

ABSTRACT: Despite significant advances in the fabrication of bioengineered scaffolds for tissue engineering, delivery of nutrients in complex engineered human tissues remains a challenge. By taking advantage of the similarities in the vascular structure of plant and animal tissues, we developed decellularized plant tissue as a prevascularized scaffold for tissue engineering applications. Perfusion-based decellularization was modified for different plant species, providing different geometries of scaffolding. After decellularization, plant scaffolds remained patent and able to transport microparticles. Plant scaffolds were recellularized with human endothelial cells that colonized the inner surfaces of plant vasculature. Human mesenchymal stem cells and human pluripotent stem cell derived cardiomyocy

SUBMITTER: Gershlak JR 

PROVIDER: S-EPMC5388455 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature