Purification, characterization and thermostability improvement of xylanase from Bacillus amyloliquefaciens and its application in pre-bleaching of kraft pulp.

ABSTRACT: Xylanases have important industrial applications but are most extensively utilized in the pulp and paper industry as a pre-bleaching agent. We characterized a xylanase from Bacillus amyloliquefaciens strain SK-3 and studied it for kraft pulp bleaching. The purified enzyme had a molecular weight of ~50 kDa with optimal activity at pH 9.0 and 50 °C. The enzyme showed good activity retention (85%) after 2 h incubation at 50 °C and pH 9.0. This enzyme obeyed Michaelis-Menten kinetics with regard to beechwood xylan with K _m and V _max values of 5.6 mg/ml, 433 μM/min/mg proteins, respectively. The enzyme activity was stimulated by Mn²⁺, Ca²⁺ and Fe²⁺ metal ions. Further, it also showed good tolerance to phenolics (2 mM) in the presence of syr

SUBMITTER: Kumar S 

PROVIDER: S-EPMC5388660 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature