Transcription Impacts the Efficiency of mRNA Translation via Co-transcriptional N6-adenosine Methylation.

ABSTRACT: Transcription and translation are two main pillars of gene expression. Due to the different timings, spots of action, and mechanisms of regulation, these processes are mainly regarded as distinct and generally uncoupled, despite serving a common purpose. Here, we sought for a possible connection between transcription and translation. Employing an unbiased screen of multiple human promoters, we identified a positive effect of TATA box on translation and a general coupling between mRNA expression and translational efficiency. Using a CRISPR-Cas9-mediated approach, genome-wide analyses, and in vitro experiments, we show that the rate of transcription regulates the efficiency of translation. Furthermore, we demonstrate that m⁶A modification of mRNAs is co-transcriptional and depends

SUBMITTER: Slobodin B 

PROVIDER: S-EPMC5388891 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature