Determining the presence of asthma-related molecules and salivary contamination in exhaled breath condensate.

ABSTRACT:

Background

Researchers investigating lung diseases, such as asthma, have questioned whether certain compounds previously reported in exhaled breath condensate (EBC) originate from saliva contamination. Moreover, despite its increasing use in 'omics profiling studies, the constituents of EBC remain largely uncharacterized. The present study aims to define the usefulness of EBC in investigating lung disease by comparing EBC, saliva, and saliva-contaminated EBC using targeted and untargeted mass spectrometry and the potential of metabolite loss from adsorption to EBC sample collection tubes.

Methods

Liquid chromatography mass spectrometry (LC-MS) was used to analyze samples from 133 individuals from three different cohorts. Levels of amino acids and eicosanoids, two classes of

SUBMITTER: Cruickshank-Quinn C 

PROVIDER: S-EPMC5389118 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature