Synthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing.

ABSTRACT: High-throughput sequencing of 16S rRNA gene amplicons (16S-seq) has become a widely deployed method for profiling complex microbial communities but technical pitfalls related to data reliability and quantification remain to be fully addressed. In this work, we have developed and implemented a set of synthetic 16S rRNA genes to serve as universal spike-in standards for 16S-seq experiments. The spike-ins represent full-length 16S rRNA genes containing artificial variable regions with negligible identity to known nucleotide sequences, permitting unambiguous identification of spike-in sequences in 16S-seq read data from any microbiome sample. Using defined mock communities and environmental microbiota, we characterized the performance of the spike-in standards and demonstrated their utility fo

SUBMITTER: Tourlousse DM 

PROVIDER: S-EPMC5389483 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature