A structure-function analysis of the yeast Elg1 protein reveals the importance of PCNA unloading in genome stability maintenance.

ABSTRACT: The sliding clamp, PCNA, plays a central role in DNA replication and repair. In the moving replication fork, PCNA is present at the leading strand and at each of the Okazaki fragments that are formed on the lagging strand. PCNA enhances the processivity of the replicative polymerases and provides a landing platform for other proteins and enzymes. The loading of the clamp onto DNA is performed by the Replication Factor C (RFC) complex, whereas its unloading can be carried out by an RFC-like complex containing Elg1. Mutations in ELG1 lead to DNA damage sensitivity and genome instability. To characterize the role of Elg1 in maintaining genomic integrity, we used homology modeling to generate a number of site-specific mutations in ELG1 that exhibit different PCNA unloading capabilities. We sho

SUBMITTER: Shemesh K 

PROVIDER: S-EPMC5389545 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature