Combining ATAC-seq with nuclei sorting for discovery of cis-regulatory regions in plant genomes.

ABSTRACT: Chromatin structure plays a pivotal role in facilitating proper control of gene expression. Transcription factor (TF) binding of cis-elements is often associated with accessible chromatin regions. Therefore, the ability to identify these accessible regions throughout plant genomes will advance understanding of the relationship between TF binding, chromatin status and the regulation of gene expression. Assay for Transposase Accessible Chromatin sequencing (ATAC-seq) is a recently developed technique used to map open chromatin zones in animal genomes. However, in plants, the existence of cell walls, subcellular organelles and the lack of stable cell lines have prevented routine application of this technique. Here, we describe an assay combining ATAC-seq with fluorescence-activated nuclei sor

SUBMITTER: Lu Z 

PROVIDER: S-EPMC5389718 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature