Exercise in vivo marks human myotubes in vitro: Training-induced increase in lipid metabolism.

ABSTRACT:

Background and aims

Physical activity has preventive as well as therapeutic benefits for overweight subjects. In this study we aimed to examine effects of in vivo exercise on in vitro metabolic adaptations by studying energy metabolism in cultured myotubes isolated from biopsies taken before and after 12 weeks of extensive endurance and strength training, from healthy sedentary normal weight and overweight men.

Methods

Healthy sedentary men, aged 40-62 years, with normal weight (body mass index (BMI) < 25 kg/m2) or overweight (BMI ≥ 25 kg/m2) were included. Fatty acid and glucose metabolism were studied in myotubes using [14C]oleic acid and [14C]glucose, respectively. Gene and protein expressions, as well as DNA methylation were measured for selected genes.

Results

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SUBMITTER: Lund J 

PROVIDER: S-EPMC5389842 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature