Proprotein convertases generate a highly functional heterodimeric form of thymic stromal lymphopoietin in humans.

ABSTRACT:

Rationale

Thymic stromal lymphopoietin (TSLP) is known to be elevated and truncated in nasal polyps (NPs) of patients with chronic rhinosinusitis and might play a significant role in type 2 inflammation in this disease. However, neither the structure nor the role of the truncated products of TSLP has been studied.

Objective

We sought to investigate the mechanisms of truncation of TSLP in NPs and the function of the truncated products.

Methods

We incubated recombinant human TSLP with NP extracts, and determined the protein sequence of the truncated forms of TSLP using Edman protein sequencing and matrix-assisted laser desorption/ionization-time of flight mass spectrometry. We investigated the functional activity of truncated TSLP using a PBMC-based bioassay.

Results

SUBMITTER: Poposki JA 

PROVIDER: S-EPMC5389936 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature