A Role of BK Channel in Regulation of Ca²⁺ Channel in Ventricular Myocytes by Substrate Stiffness.

ABSTRACT: Substrate stiffness is crucial for diverse cell functions, but the mechanisms conferring cells with mechanosensitivity are still elusive. By tailoring substrate stiffness with 10-fold difference, we showed that L-type voltage-gated Ca²⁺ channel current density was greater in chick ventricular myocytes cultured on the stiff substrate than on the soft substrate. Blockage of the BK channel increased the Ca²⁺ current density on the soft substrate and consequently eliminated substrate stiffness regulation of the Ca²⁺ channel. The expression of the BK channel, including the STREX-containing α-subunit that forms stretch-activated BK channel in myocytes and the BK channel function in myocytes (and also in HEK293 cells heterologously expressing STREX-containing α- a

SUBMITTER: Zhao H 

PROVIDER: S-EPMC5389963 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature