Anaerobicity prepares Saccharomyces cerevisiae cells for faster adaptation to osmotic shock.

ABSTRACT: Yeast cells adapt to hyperosmotic shock by accumulating glycerol and altering expression of hundreds of genes. This transcriptional response of Saccharomyces cerevisiae to osmotic shock encompasses genes whose products are implicated in protection from oxidative damage. We addressed the question of whether osmotic shock caused oxidative stress. Osmotic shock did not result in the generation of detectable levels of reactive oxygen species (ROS). To preclude any generation of ROS, osmotic shock treatments were performed in anaerobic cultures. Global gene expression response profiles were compared by employing a novel two-dimensional cluster analysis. The transcriptional profiles following osmotic shock under anaerobic and aerobic conditions were qualitatively very similar. In particular, it

SUBMITTER: Krantz M 

PROVIDER: S-EPMC539022 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature