High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells.

ABSTRACT: Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes. To date, studies have been published that use small panels of iPSC-derived cell lines to study monogenic diseases. However, to study complex diseases, where the genetic variation underlying the disorder is unknown, a sizable number of patient-specific iPSC lines and controls need to be generated. Currently the methods for deriving and characterizing iPSCs are time consuming, expensive, and, in some cases, descriptive but not quantitative. Here we set out to develop a set of simple methods that reduce cost and increase throughput in the character

SUBMITTER: D'Antonio M 

PROVIDER: S-EPMC5390243 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature