Caging and Photoactivation in Single-Molecule Forster Resonance Energy Transfer Experiments.

ABSTRACT: Caged organic fluorophores are established tools for localization-based super-resolution imaging. Their use relies on reversible deactivation of standard organic fluorophores by chemical reduction or commercially available caged dyes with ON switching of the fluorescent signal by ultraviolet (UV) light. Here, we establish caging of cyanine fluorophores and caged rhodamine dyes, i.e., chemical deactivation of fluorescence, for single-molecule Förster resonance energy transfer (smFRET) experiments with freely diffusing molecules. They allow temporal separation and sorting of multiple intramolecular donor-acceptor pairs during solution-based smFRET. We use this "caged FRET" methodology for the study of complex biochemical species such as multisubunit proteins or nucleic acids containing more

SUBMITTER: Jazi AA 

PROVIDER: S-EPMC5390306 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature