Nested-PCR assay for detection of Schistosoma japonicum infection in domestic animals.

ABSTRACT:

Background

Schistosomiasis japonica is a common zoonosis. Domestic animals are the primary source of infection and play an important role in disease transmission. The prevalence and infectivity of this disease in domestic animals in China have significantly decreased and, for this reason, diagnostics with a higher sensitivity have become increasingly necessary. It was reported that polymerase chain reaction (PCR)-based methods could be used to detect schistosome infection in humans and animals and presented a high sensitivity and specificity. The present study aimed to develop a PCR-based method for detection of Schistosoma japonicum infection in domestic animals.

Methods

A specific nested-PCR assay was developed to detect S. japonicum infection in domestic animals via ampli

SUBMITTER: Zhang X 

PROVIDER: S-EPMC5390378 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature