Resveratrol reduces the apoptosis induced by cigarette smoke extract by upregulating MFN2.

ABSTRACT:

Purpose

The aim of this study was to investigate the effect of resveratrol (RSV) on cigarette smoke extract (CSE)-induced cell apoptosis and mitochondrial dysfunction in vitro, as well as changes in the MFN2 expression level.

Methods

Cultured human bronchial epithelial (HBE) cells were initially treated with CSE to induce apoptosis, followed by incubation either with or without RSV. Numerous techniques were used to evaluate the outcomes of the present study, including a cell counting kit-8 assay, real-time quantitative polymerase chain reaction (real-time qPCR), western blotting, JC-1 fluorescence, Hoechst 33342 staining, Annexin V-PI flow cytometry apoptosis analyses, and siRNA technology.

Results

A 24 h incubation in 3.5% CSE induced apoptosis in HBE cells, and pret

SUBMITTER: Song C 

PROVIDER: S-EPMC5391199 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature