Lesion complexity drives age related cancer susceptibility in human mammary epithelial cells.

ABSTRACT: Exposures to various DNA damaging agents can deregulate a wide array of critical mechanisms that maintain genome integrity. It is unclear how these processes are impacted by one's age at the time of exposure and the complexity of the DNA lesion. To clarify this, we employed radiation as a tool to generate simple and complex lesions in normal primary human mammary epithelial cells derived from women of various ages. We hypothesized that genomic instability in the progeny of older cells exposed to complex damages will be exacerbated by age-associated deterioration in function and accentuate age-related cancer predisposition. Centrosome aberrations and changes in stem cell numbers were examined to assess cancer susceptibility. Our data show that the frequency of centrosome aberrations proport

SUBMITTER: Sridharan DM 

PROVIDER: S-EPMC5391225 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature