A nondestructive method to estimate the chlorophyll content of Arabidopsis seedlings.

ABSTRACT:

Background

Chlorophyll content decreases in plants under stress conditions, therefore it is used commonly as an indicator of plant health. Arabidopsis thaliana offers a convenient and fast way to test physiological phenotypes of mutations and treatments. However, chlorophyll measurements with conventional solvent extraction are not applicable to Arabidopsis leaves due to their small size, especially when grown on culture dishes.

Results

We provide a nondestructive method for chlorophyll measurement whereby the red, green and blue (RGB) values of a color leaf image is used to estimate the chlorophyll content from Arabidopsis leaves. The method accommodates different profiles of digital cameras by incorporating the ColorChecker chart to make the digital ne

SUBMITTER: Liang Y 

PROVIDER: S-EPMC5391588 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature