Identification and functional characterisation of a Schistosoma japonicum insulin-like peptide.

ABSTRACT:

Background

Previous studies have shown that insulin receptors in schistosomes, triggered by host insulin, play an important role in parasite growth, development and fecundity by regulating glucose metabolism. However, limited information is available on the recently identified endogenous insulin-like peptide (ILP) in blood flukes.

Results

We isolated ILPs from Schistosoma japonicum (SjILP) and S. recognised (SmILP) and present results of their molecular and structural analysis. SjILP shares 63% amino acid identity with SmILP, but only 18% identity with human insulin. There is high cross immunological reactivity between the S. japonicum and S. mansoni ILPs as observed in western blots using an anti-SjILP polyclonal antibody. ADP binding/hydrolysis ability was observed in both

SUBMITTER: Du X 

PROVIDER: S-EPMC5391603 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature