Role of glycine 221 in catalytic activity of hyaluronan-binding protein 2.

ABSTRACT: HABP2 (hyaluronan-binding protein 2) is a Ca²⁺-dependent serine protease with putative roles in blood coagulation and fibrinolysis. A G221E substitution, known as the Marburg I polymorphism, reportedly affects HABP2 function and has been associated with increased risk for cardiovascular disease. However, the importance of Gly-221 for HABP2 activity is unclear. Here, we used G221E, G221A, and G221S mutants to assess the role of Gly-221 in HABP2 catalysis. The G221E variant failed to activate the single-chain urokinase-type plasminogen activator, and the G221A and G221S variants displayed moderately reduced single-chain urokinase-type plasminogen activator activation. Activity toward the peptide substrate S-2288 was markedly decreased in all HABP2 variants, with G221E being the mo

SUBMITTER: Stavenuiter F 

PROVIDER: S-EPMC5391765 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature