Multicolour localization microscopy by point-spread-function engineering.

ABSTRACT: Super-resolution microscopy has revolutionized cellular imaging in recent years^1-4. Methods relying on sequential localization of single point emitters enable spatial tracking at ~10-40 nm resolution. Moreover, tracking and imaging in three dimensions is made possible by various techniques, including point-spread-function (PSF) engineering^5-9 -namely, encoding the axial (z) position of a point source in the shape that it creates in the image plane. However, a remaining challenge for localization-microscopy is efficient multicolour imaging - a task of the utmost importance for contextualizing biological data. Normally, multicolour imaging requires sequential imaging^{10, 11}, multiple cameras¹², or segmented dedicated fields of view^{13, 14}.

SUBMITTER: Shechtman Y 

PROVIDER: S-EPMC5391844 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature