Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections.

ABSTRACT:

Objective

Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome.

Methods

S aureus isolates derived from LRTIs and control groups (nasal carriage and bacteraemia) were genotyped using StaphyType DNA microarray. Agr activity was evaluated using the CAMP synergistic haemolysis assay and the Vesicle Lysis Test (VLT). Discordant strains were analysed by quantitative reverse-transcriptase real-time PCR (qRT-PCR).

Results

Agr was functional in 79.7% and 84.5% of strains according to the CAMP and VLT assays respectively. Higher concordance with RNAIII

SUBMITTER: Gomes-Fernandes M 

PROVIDER: S-EPMC5391941 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature