New insights into valve-related intramural and intracellular bacterial diversity in infective endocarditis.

ABSTRACT:

Aims

In infective endocarditis (IE), a severe inflammatory disease of the endocardium with an unchanged incidence and mortality rate over the past decades, only 1% of the cases have been described as polymicrobial infections based on microbiological approaches. The aim of this study was to identify potential biodiversity of bacterial species from infected native and prosthetic valves. Furthermore, we compared the ultrastructural micro-environments to detect the localization and distribution patterns of pathogens in IE.

Material and methods

Using next-generation sequencing (NGS) of 16S rDNA, which allows analysis of the entire bacterial community within a single sample, we investigated the biodiversity of infectious bacterial species from resected native and prosthetic valves

SUBMITTER: Oberbach A 

PROVIDER: S-EPMC5391965 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature