Lipid Droplet Biogenesis and Function in the Endothelium.

ABSTRACT:

Rationale

Fatty acids (FA) are transported across the capillary endothelium to parenchymal tissues. However, it is not known how endothelial cells (EC) from large vessels process a postprandial surge of FA.

Objective

This study was designed to characterize lipid droplet (LD) formation in EC by manipulating pathways leading to the formation and degradation of LD. In addition, several functions of LD-derived FA were assessed.

Methods and results

LD were present in EC lining the aorta after the peak in plasma triglycerides initiated by a gavage of olive oil in mice, in vivo. Similarly, in isolated aorta, oleic acid treatment generates LD in EC ex vivo. Cultured EC readily form LD largely via the enzyme DGAT (diacylglycerol O-acyltransferase 1) and degrade LD via ATGL (ad

SUBMITTER: Kuo A 

PROVIDER: S-EPMC5392152 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature