Integration of high-risk human papillomavirus into cellular cancer-related genes in head and neck cancer cell lines.

ABSTRACT:

Background

Human papillomavirus (HPV)-positive oropharyngeal cancer is generally associated with excellent response to therapy, but some HPV-positive tumors progress despite aggressive therapy. The purpose of this study was to evaluate viral oncogene expression and viral integration sites in HPV16- and HPV18-positive squamous cell carcinoma lines.

Methods

E6/E7 alternate transcripts were assessed by reverse transcriptase-polymerase chain reaction (RT-PCR). Detection of integrated papillomavirus sequences (DIPS-PCR) and sequencing identified viral insertion sites and affected host genes. Cellular gene expression was assessed across viral integration sites.

Results

All HPV-positive cell lines expressed alternate HPVE6/E7 splicing indicative of active viral oncogenesis.

SUBMITTER: Walline HM 

PROVIDER: S-EPMC5392184 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature