Transcriptome Analysis of Orange Head Chinese Cabbage (Brassica rapa L. ssp. pekinensis) and Molecular Marker Development.

ABSTRACT: Due to the visual appearance and high carotenoid content, orange inner leaves are a desirable trait for the Chinese cabbage. To understand the molecular mechanism underlying the formation of orange inner leaves, the BrCRTISO (Bra031539) gene, as the Br-or candidate gene, was analyzed among the white and orange varieties, and 7 single nucleotide polymorphisms (SNPs) were identified. However, only one SNP (C⁹⁵² to T⁹⁵²) altered the amino acid sequence, resulting in a mutation from Leu³¹⁸ to Phe³¹⁸ in the orange varieties. Additionally, we analyzed differentially expressed genes (DEGs) between the orange and white F₂ individuals (14-401 × 14-490) and found four downregulated genes were involved in the carotenoid biosynthesis

SUBMITTER: Li J 

PROVIDER: S-EPMC5392394 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature