Antigen-Presenting Human γδ T Cells Promote Intestinal CD4⁺ T Cell Expression of IL-22 and Mucosal Release of Calprotectin.

ABSTRACT: The cytokine IL-22 plays a critical role in mucosal barrier defense, but the mechanisms that promote IL-22 expression in the human intestine remain poorly understood. As human microbe-responsive Vγ9/Vδ2 T cells are abundant in the gut and recognize microbiota-associated metabolites, we assessed their potential to induce IL-22 expression by intestinal CD4⁺ T cells. Vγ9/Vδ2 T cells with characteristics of APCs were generated from human blood and intestinal organ cultures, then cocultured with naive and memory CD4⁺ T cells obtained from human blood or the colon. The potency of blood and intestinal γδ T-APCs was compared with that of monocytes and dendritic cells, by assessing CD4⁺ T cell phenotypes and proliferation as well as cytokine and transcription factor

SUBMITTER: Tyler CJ 

PROVIDER: S-EPMC5392732 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature