Reference genes for normalization of qPCR assays in sugarcane plants under water deficit.

ABSTRACT:

Background

Sugarcane (Saccharum spp.) is the main raw material for sugar and ethanol production. Among the abiotic stress, drought is the main one that negatively impact sugarcane yield. Although gene expression analysis through quantitative PCR (qPCR) has increased our knowledge about biological processes related to drought, gene network that mediates sugarcane responses to water deficit remains elusive. In such scenario, validation of reference gene is a major requirement for successful analyzes involving qPCR.

Results

In this study, candidate genes were tested for their suitable as reference genes for qPCR analyses in two sugarcane cultivars with varying drought tolerance. Eight candidate reference genes were evaluated in leaves sampled in plants subjected to water

SUBMITTER: de Andrade LM 

PROVIDER: S-EPMC5392966 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature