Identification of proteins in laser-microdissected small cell numbers by SELDI-TOF and Tandem MS.

ABSTRACT:

Background

Laser microdissection allows precise isolation of specific cell types and compartments from complex tissues. To analyse proteins from small cell numbers, we combine laser-microdissection and manipulation (LMM) with mass spectrometry techniques.

Results

Hemalaun stained mouse lung sections were used to isolate 500-2,000 cells, enough material for complex protein profiles by SELDI-TOF MS (surface enhanced laser desorption and ionization/time of flight mass spectrometry), employing different chromatographic ProteinChip Arrays. Initially, to establish the principle, we identified specific protein peaks from 20,000 laser-microdissected cells, combining column chromatography, SDS-PAGE, tryptic digestion, SELDI technology and Tandem MS/MS using a ProteinChip Tandem MS In

SUBMITTER: Kwapiszewska G 

PROVIDER: S-EPMC539305 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature